Climate change activists climbed on a train and a campaigner glued himself to the carriage in the third day of protests aiming to “shut down London”.
Extinction Rebellion protesters have been blocking traffic at Marble Arch, Waterloo Bridge, Parliament Square and Oxford Circus since Monday.
Wednesday’s action – affecting a Docklands Light Railway (DLR) train at Canary Wharf – is causing minor delays.
Nearly 300 people have been arrested this week over the protests.
Most have been arrested in connection to public order offences.
The Met Police said “contingency plans are in place should custody suites become full”.
The group targeted the DLR after it changed its mind about disrupting the Tube network on Wednesday.
A man and a woman unfurled a banner on top of a carriage while another man glued his hand to the train window.
A spokesman for TfL said: “There are minor delays on the DLR between Bank and Lewisham because two protesters have climbed on a train at Canary Wharf.”
Earlier British Transport Police ordered Transport for London (TfL) to switch off wi-fi at Tube stations in an attempt to deter protests.
Supt Matt Allingham said extra officers would be on duty throughout the day, adding: “We will not tolerate any activity which disrupts the millions of passengers who rely on using the rail network in London.”
Extinction Rebellion said “thousands” more people were willing to be arrested as part of the non-violent disruption.
It had been planning to target London Underground to “highlight the emergency of ecological collapse” and persuade ministers to meet group members.
London Mayor Sadiq Khan urged protesters to “think again”, adding public transport helps to tackle climate change and Tube disruption could “risk Londoners’ safety”.
On Wednesday morning, the campaign said: “Today we will disrupt one overground line as part of our escalating campaign to demand the government acts now on the climate and ecological emergency.”
Protester and climate lawyer Farhana Yamin, who was arrested on Tuesday, told BBC Radio 4’s Today programme: “I totally want to apologise to people using public transport.
“But at the same time we need to take actions that are disruptive so everyone understands the dangers we’re facing right now. People should understand that we are at a critical moment in our humanity’s history.”
Jace Tyrrell, chief executive of the New West End Company, said the first two days of protests had so far caused a loss of £12m in trade in the West End.
He told BBC Radio London: “Everyone has a right to peaceful protest… But this is really disruptive for us.”
In Edinburgh, dozens of people were arrested when hundreds of protesters blocked a main road.
Organisers said protests had been held in more than 80 cities across 33 countries and wanted to continue “shutting down London” until 29 April.
Campaigners at Waterloo Bridge and Oxford Circus have been ordered to restrict their protests to Marble Arch after they caused widespread disruption on Monday. That order will continue until 21:10 on Friday.
Joao Verga, who works at a printing company, says the protesters at Oxford Circus have caused him disruption.
The 58-year-old said: “We’re trying to make a living and we can’t. We move very quick in this country, this is only going to slow down everything.”
Three men and two women, in their 40s and 50s, arrested on suspicion of criminal damage at Shell’s headquarters in London on Monday, have since been released while inquiries continue.
The majority of the other protesters detained have been held on suspicion of public order offences.
What is Extinction Rebellion?
Since the group was set up last year, members have shut bridges, poured buckets of fake blood outside Downing Street, blockaded the BBC and stripped semi-naked in Parliament.
It has three core demands: for the government to “tell the truth about climate change”, reduce carbon emissions to zero by 2025, and create a citizens’ assembly to oversee progress.
Controversially, the group is trying to get as many people arrested as possible.
But critics say they cause unnecessary disruption and waste police time when forces are already overstretched.
The government said it shared “people’s passion” to combat climate change and “protect our planet for future generations”.
The Department for Business, Energy and Industrial Strategy said the UK had cut its emissions by 44% since 1990.
A spokesman said: “We’ve asked our independent climate experts for advice on a net zero emissions target and set out plans to transition to low emission vehicles and significantly reduce pollution through our Clean Air Strategy.”